Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
FIGURE: 1 / 2
BTE 004-09-02 detects Pregnancy-associated plasma protein A (PAPP-A) from human samples. Heat and SDS denatured PAPP-A is also recognized, but not acid treated PAPP-A.
BTE 004-09-02 has been successfully used in ELISA, Western blotting and Immunohistochemistry procedures. In sandwich ELISA the optimal combination is BTE 004-09-02 used as coating antibody and biotinylated ABS 006-24-02 used as detection antibody. BTE 004-09-02 can also be used as detection antibody in sandwich ELISA in combination with a polyclonal antibody towards PAPP-A in coat. BTE 004-09-02 detect both reduced and unreduced PAPP-A in Western Blotting, though reaction to unreduced is less than that of ABS 006-01-02 and ABS 006-24-02.
The epitope of BTE 004-09-02 is different from ABS 006-01-02 and ABS 006-24-02. The epitope is located on the PAPP-A subunit of the heterotetramer.
NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL
Pregnancy-associated plasma protein-A (PAPP-A, Insulin-like growth factor binding protein-4 protease, PAPP-A-proMBP complex) is a large heterottetrameric glycoprotein of approximately 500 kDa, which was first discovered in serum from pregnant women. The hetreotetramer consists of two PAPP-A subunits and two proMBP subunits. Low maternal serum levels of PAPP-A in first trimester biochemical screening is used as a marker of Down's syndrome (trisomy 21).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: aspecific BCL2 ARE-binding protein 2; differentially placenta 1 expressed protein; IGF-dependent IGFBP-4 protease; IGFBP-4 ase; insulin-like growth factor-dependent IGF binding protein-4 protease; Insulin-like growth factor-dependent IGF-binding protein 4 protease; PAPP A; PAPP-A; Pappalysin; Pappalysin-1; pregnacy-associated plasma protein A; Pregnancy-associated plasma protein A; pregnancy-associated plasma protein A, pappalysin 1; RP11-45A16.1
Gene Aliases: ASBABP2; DIPLA1; IGFBP-4ase; PAPA; PAPP-A; PAPPA; PAPPA1
UniProt ID: (Human) Q13219
Entrez Gene ID: (Human) 5069
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn moreGet expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support